Miadlikowska et al. (2014) and Magain et al. (2017) developed primers for the EF2.1 locus. All three primers below were designed for studies on Peltigera.
EFT2-1F (forward: 5′-AAYATGWSBGTBATYGC-3′) Miadlikowska et al. (2014)
EFT2-4R (reverse: 5′-GGVACCATYTTVGARAC-3′) Miadlikowska et al. (2014)
EFT2.1_3R (reverse: 5′-ATCCCTGATACCAATGCATGCC-3′) Magain et al. (2017)
References
Magain, N., Miadlikowska, J., Goffinet, B., Sérusiaux, E. and Lutzoni, F. 2017. Macroevolution of Specificity in Cyanolichens of the Genus Peltigera Section Polydactylon (Lecanoromycetes, Ascomycota). Systematic Biology 66(1): 74–99.
Miadlikowska, J., Richardson, D., Magain, N., Ball, B., Anderson, F., Cameron, R., Lendemer, J., Truong, C. and Lutzoni, F. 2014. Phylogenetic placement, species delimitation, and cyanobiont identity of endangered aquatic Peltigera species (lichen-forming Ascomycota, Lecanoromycetes). American Journal of Botany 101: 1141–1156.